Sorry about that and thanks for your help! I am using Graphtec Pro Studio 2.21 and Graphtec Pro Studio Production Manager 2.21, running on Windows 7.
Are there specific settings within Graphtec Pro Studio or Production Manager that would be helpful for me to share screenshots of?
(Yep, tried contacting Graphtec support.)
Edit: I have determined that the files are printing out at a scale exactly .25 of the designs. As a workaround, I can increase the scale 400%, but would love to figure out what is causing the scale to shrink so I can fix it properly!